MAP1LC3A and infection: To determine whether a specific autophagic response is induced in host epithelial cells during infection by C. albicans, we first measured the autophagic flux by measuring LC3-II turnover by Western blot using the autophagy flux inhibitor Bafilomycin A1.25 During the activation of the autophagic process, cytosolic LC3 (termed LC3-I) is conjugated with PE and the amount of this lipidated form of LC3, termed LC3-II, correlates with the number of autophagosomes.