In the multivariate analysis, presence of evident liver cirrhosis (p < 0.001), intermediate-advanced stage HCC (p < 0.001), higher alanine aminotransferase level (p = 0.049), higher AFP level (p = 0,049), higher Child-Pugh score (p < 0.001), and cause of cirrhosis other than virus-related hepatitis (p = ≤ 0.001) were significant predictive factors for AD development. The gene discussed is AFP; the disease is Cirrhosis.