In the case of ZBTB18, we recently combined several approaches to establish the biomolecular, biochemical, and neurobiological impacts of two such variants, namely NP_991331.1:p.Asn461Ser (N461S) ([Farwell, Shahmirzadi, El‐Khechen, et al., 2015]; rs797044885) detected in an individual with microcephaly, as well as NP_991331.1:p.Arg495Gly (R495G) (Rauch et al., 2012) detected in an individual with macrocephaly. This evidence concerns the gene ZBTB18 and microcephaly.