PIWIL1 and Miyoshi myopathy: Supplementary Figure 1 shows the efficiency of transfection. Results of the CCK-8 assay showed that PIWIL1 downregulation caused a significant decrease in cell viability, and PIWIL1 overexpression resulted in higher cell viability compared with that in the NC group (Figures 2A, B), suggesting that PIWIL1 promotes the proliferation of MM.