The results illustrated that COL5A1 was upregulated in 13 cancers, including breast invasive carcinoma (BRCA), cholangiocarcinoma (CHOL), COAD, esophageal carcinoma (ESCA), GBM, HNSC, kidney renal clear cell carcinoma (KIRC), LIHC, lung squamous cell carcinoma (LUSC), lung adenocarcinoma (LUAD), rectal adenocarcinoma (READ), STAD, and thyroid carcinoma (THCA). This evidence concerns the gene COL5A1 and squamous cell lung carcinoma.