In order to study the role of ghrelin in the pathogenesis of heart failure, we obtained the myocardial tissue of a rat model, and extracted the protein in the tissue, and with the help of a series of methods to detect the possible molecular mechanism of ghrelin from the level of protein and RNA, such as immunohistochemistry, Western blot, real-time quantitative PCR, and other methods. This evidence concerns the gene GHRL and heart failure.