NVP-BEZ235 and PP242 suppressed SPOP protein expression in RCC cell lines in a dose- and time-dependent manner, coincident with reduced Akt phosphorylation, whereas no obvious change was observed in Rapamycin-treated cells after 48 h of exposure (Figure 4C; Supplementary Figure S2A). This evidence concerns the gene AKT1 and renal cell carcinoma.