Besides, the overexpression of NFAT1 reversed the PD-L1 expression inhibition induced by NFAT1 silencing in RCC cells (Supplementary Fig. 2G-2H), as well as the main downstream molecules of PD-1 checkpoint pathway, DCLRE1A and MAP 3 K20 (Supplementary Fig. 2I). The gene discussed is NFATC2; the disease is renal cell carcinoma.