And through lentivirus transfection, double luciferase analysis, cell proliferation and migration/invasion examination, and cell chemoresistance detection, we further approved that miR-656-3p inhibited the proliferation and migration of colorectal cancer cells by directly targeting to Sphingosine-1-phosphate phosphatase 1 (SGPP1). The gene discussed is SGPP1; the disease is colorectal cancer.