Our results showed that cell lines derived from metabolically active organs such as kidney (HEK293: embryonic kidney) or liver (HepG2: liver cancer) secreted about 20-fold less asprosin into the cell culture supernatant when compared to cells derived from connective tissues such as bone (U-2 OS: bone osteosarcoma), eye (RPE: retinal pigment epithelial), lung (WI-26: fetal lung), cartilage (HCH: primary human chondrocytes derived from knee cartilage) or skin (HDF: primary human dermal fibroblasts) (Fig. 4A). This evidence concerns the gene FBN1 and bone osteosarcoma.