The accelerated differentiation phenotype at 35 days that we observed with pseudotime analysis has been described before in LRRK2-related PD.8, 9, 10 In addition, the MUT midbrain organoids have no evident differences in the cell type populations at both time points and pseudotime analysis revealed that besides the untimely differentiation, the MUT70 midbrain organoids face a premature arrest or slowdown of the differentiation capacity. The gene discussed is LRRK2; the disease is Parkinson disease.