The fact that these mutants attenuate NF-κB nuclear translocation at late infection times (Fig 6) and that all of them modify D-Ala to alaninol in fourth position of stem peptides (S7 Fig), led us to propose this unprecedented modification as responsible for diminishing the immuno-stimulatory level of the PG of intracellular bacteria. The gene discussed is NFKB1; the disease is infection.