At 2 h after infection with the wt strain, the colocalization of the FCP with the early endosomal marker EEA1 was only 5.4% (Fig 6A and 6C) and with LAMP-1 8.51% (Fig 6D), indicating that the majority of F. novicida were either in the cytosol or in a compartment lacking these markers. The gene discussed is EEA1; the disease is infection.