STAT3 and breast neoplasm: To determine whether there are differences between the kinetics of STAT3 mRNA suppression in primary murine syngeneic breast tumors by Chol-siSTAT3 and Chol-DsiSTAT3 polyplexes after IV administration, we intravenously administered a single equimolar dose of Chol-siSTAT3 or Chol-DsiSTAT3 complexed with PLL[30]-PEG[5K] at the indicated N/P ratio and compared normalized STAT3 mRNA copy numbers in early-stage primary murine 4T1 breast tumors to vehicle alone every 24 h over 96 h by RT-ddPCR (Figure 3B).