To determine whether the activities of the 5′-overlapping siSTAT3 and DsiSTAT3 sequences against STAT3 mRNA are similar in murine syngeneic breast cancer epithelial cells, we electroporated 4T1 cells with equimolar concentrations of siSTAT3, DsiSTAT3 (a 3′-extension of the siSTAT3 sequence that suppresses 81% of murine STAT3 mRNA in 4T1 cells 24 h after electroporation at 300 nM [2]), inactive siCTRL, or inactive DsiCTRL and compared normalized murine STAT3 mRNA copy numbers to electroporated 4T1 cells over 72 h with RT-ddPCR (Figure 2A). Here, STAT3 is linked to breast cancer.