By comparing the gene expression profiles of organoids, we showed that a series of signaling pathways varied across normal gallbladder, GBA and GBC organoids, for example, JAK‐STAT, PI3K‐AKT, oxidative phosphorylation and TP53 pathways, which are implicated in cell proliferation ability and tumoural malignancy. This evidence concerns the gene SOAT1 and neoplasm.