By comparing the gene expression profiles of organoids, we showed that a series of signaling pathways varied across normal gallbladder, GBA and GBC organoids, for example, JAK‐STAT, PI3K‐AKT, oxidative phosphorylation and TP53 pathways, which are implicated in cell proliferation ability and tumoural malignancy. The gene discussed is TP53; the disease is neoplasm.