Overall, our study suggests that recombinant adenoviruses co‐expression of ING4 and OSM that in vitro, Ad‐ING4‐OSM significantly inhibited the growth, enhanced apoptosis, altered cell cycle with G1 and G2/M phase arrest and downregulated the expression of P21, P27, P53 and survivin in laryngeal cancer Hep‐2 cells. Here, OSM is linked to laryngeal carcinoma.