To test for a potential influence of TGFβ1-mediated pEMT on stemness characteristics of TKA organoids, we therefore analyzed TGFβ1-induced changes in the expression of the ISC signature and of Cd44, Cd133, Aldh1a1, Epcam, Pou5f1 (Oct4), Nanog, and Sox2 which represent the murine orthologues of human CRC stem cell markers [54, 55]. Here, NANOG is linked to colorectal carcinoma.