However, loss of Nf2 has not been shown to be sufficient to cooperate with Ras and drive tumor formation independent of other accessory mutations and whereas loss of Nf2 has been previously shown to promote the formation of mixed HCC and ICC when it is deleted from the liver during development (47), it was unclear whether de novo somatic Nf2 mutations interact with other ICC-relevant mutations in vivo. This evidence concerns the gene NF2 and neoplasm.