ROBO1 and breast cancer: In our study, we found that FUS was upregulated in BC and could bind to the downstream intron of pre-ROBO1, the precursor of circROBO1, to promote the back splicing of pre-ROBO1 to form the mature circROBO1, as demonstrated by insertion of an HA tag into the first exon of cricROBO1 and consequent RNA pulldown assays.