hMDMs infected with Listeria expressing the SPI-1 T3SS inner rod protein PrgJ induced robust inflammasome activation, indicated by significantly increased IL-18 secretion as well as robust IL-1α and IL-1β secretion compared to mock infection or WT Lm infection alone (Figs 7A and S10), in agreement with our previous findings [45]. This evidence concerns the gene CHGA and infection.