Among 96 newborn infants diagnosed with PS by echocardiography from October 2014 to December 2020 at Xinhua Hospital, 33 had serum NT-proBNP levels measured over 48 h after birth, the remaining 63 patients had a gestational age over 34 weeks, 1 had PS and VSD, 2 had PA, 11 had other complex congenital heart diseases (1 with right ventricular diverticulum, 4 with double outlet right ventricle, 3 with complete transposition of the great artery, 1 with single ventricle, 1 with aortic stenosis, and 2 with severe TR), 1 had severe pleural effusion and ascites, and 1 had missing data. The gene discussed is NPPB; the disease is Pleural effusion.