Through the enrichment analysis of KEGG and GSEA pathways, we noticed that ZCCHC17 is mainly involved in the body’s immune system in terms of mechanism regulation and regulating a series of immunotherapy-related processes, including cancer immunotherapy by PD-1 blockade, PD-L1 expression and PD-1 checkpoint pathway in cancer, these analyses emphasize the link between ZCCHC17 and immune response. Here, CD274 is linked to cancer.