The ROC curves of EpCAM protein expression level depict significant discriminatory power between HCC patients and non-HCC subjects (AUC = 0.92, CI = 0.87-0.97, P < 0.0001), as shown in Figure S2, suggesting comparable diagnostic accuracy to that of AFP (AUC = 0.961, CI = 0.92-0.99, P < 0.0001). This evidence concerns the gene AFP and hepatocellular carcinoma.