The frequency of PIK3CA mutations was 6% (41/711), and 80% (33/41) of PIK3CA-mutated CCA tumors were driver missense mutations (T1025A, E365K, E545K, E542K, C420R, Q546K, C901F, R38C, N345K, R88Q, R108H, M1043I, K111E, H1047R and H1047L) (Figs. 1–2, 3A and Table S1). This evidence concerns the gene PIK3CA and cholangiocarcinoma.