Even though, ARID1A-silencing in CCA cell lines minimally increased phosphorylation of AKT at Ser-473 (pAKTS473), treatment with MK-2206 (30 μM) significantly reduced pAKTS473 level in ARID1A-knockdown KKU-213A and HUCCT-1 cell lines compared to the non-targeting control shRNA (Figs. 8A–8C). Here, ARID1A is linked to cholangiocarcinoma.