In Nepal, cotrimoxazole prophylaxis is given to HIV-infected adults with a CD4 count of <500 cells/μl, adults who have had an episode of Pneumocystis carinii pneumonia and individuals with symptomatic HIV infection [47].Comorbidities in PLHIV attending the ART center and people were not assessed in this study, and all the subjects were screened only once for S. aureus or MRSA colonization. This evidence concerns the gene CD4 and pneumocystosis.