Immunohistochemical staining revealed that lentivirus infection was successful in the NPTX2 overexpression group, while NPTX2 was highly expressed in DU145 cells (Figure 5D), and there was less positive expression of Ki67 in NPTX2-overexpressing tumors than in control tumors (Figure 5E). This evidence concerns the gene NPTX2 and infection.