Further , through exploring the correlation between GDF15 and DUSP4 in ESCC samples from TCGA, we found that there is a positive relationship between GDF15 and DUSP4 (Figure 5G).Western blot assays were further carried out to verify the expression changes of GDF15 and DUSP4 following PCAT6 knockdown in Eca-109 and Kyse-30 cells. This evidence concerns the gene PCAT6 and esophageal squamous cell carcinoma.