Among these HCC cell lines, Huh-7 and Hep-3B cells showed the highest and lowest TOP2A expression levels (Figure S1B and S1C), according to which Huh-7 and Hep-3B cell lines were selected to establish stable knockdown (Huh-7) and overexpression (Hep-3B) cell lines respectively (Figure S1D-S1F). This evidence concerns the gene TOP2A and hepatocellular carcinoma.