Based on the above selected mRNA structures, we optimized the SP of light chain and heavy chain sequences in the protein coding sequence region via applying three SPs which originate from human immunoglobulin kappa light chain (hIgLC), interleukin-10 (IL-10) and human IgG1 heavy chain (hIgHC) for the expression validation in AML-12 cell lines in vitro. This evidence concerns the gene IL10 and acute myeloid leukemia.