Based on the results of CheckMate 040, a study of advanced hepatocellular carcinoma treated with nivolumab, PD-L1 predictive effect was not that significant: objective response in PD-L1 positive (cut-off value was 1%) group was 26%(9/34), while in PD-L1 negative group, that was 19%(26/140) 12. This evidence concerns the gene CD274 and hepatocellular carcinoma.