Multivariate analysis showed that HGB level <110 g/L (P=0.004, OR=5.026), serum ALP >150 U/L (P=0.004, OR=2.271), presence of lymph node metastasis (P<0.001, OR=4.073) were correlated with BM occurrence in HCC. This evidence concerns the gene CYGB and metastatic malignant neoplasm in the lymph nodes.