These data clarified the importance of kinase signaling pathway in the induction of HO-1 and GCLC by butein and implied that ERK1/2 was the candidate kinase for Nrf2 activation, nuclear translocation, and subsequent HO-1 and GCLC induction by butein in both CHF and sham groups. The gene discussed is MAPK3; the disease is congestive heart failure.