Finally, expression of well-known DS smooth muscle-related genes ACTA2 (Urabe et al., 1992; Hagner et al., 2020) and TAGLN (Heitman et al., 2020) were significantly (p < 0.0001, FindAllMarkers function with implemented Wilcoxon Rank Sum test) enriched in our DS population (Figure 2F, Supplementary Figure S4A, Supplementary Table S2) among the fibroblasts populations, while MYH11 and MYL9 (Heitman et al., 2020) were not significantly enriched (Supplementary Figure S4A, Supplementary Table S2). The gene discussed is ACTA2; the disease is Dravet syndrome.