In a previous study on 73 NSCLC patients treated with anti-PD-1/PD-L1 ICI, the density of PD-L1+ Treg in the TME was indicated as an additional prediction biomarker of response to ICI (34), thus Treg warrant consideration as a therapeutic target to augment the clinical efficacy of ICI in lung cancer. This evidence concerns the gene CD274 and lung cancer.