To further determine whether CaMKII is involved in zinc deficiency-induced STAT3 activation, CaMKII inhibitor KN93 (10 uM) and its inactive analog KN92 (10 uM) were applied 2 h before exposure to TPEN for 2 h or 30 min before the onset of reoxygenation (Figures 4A,B). This evidence concerns the gene STAT3 and Zinc deficiency.