Figure 7 shows that both UUO and H2O2 activated Wnt3α/β-catenin/GSK-3β protein expression, whereas their expression was significantly inhibited by either Dapa or Wnt/β-catenin inhibitor ICG-001. This finding indicates that Dapa treatment alleviates renal fibrosis through the Wnt3α/β-catenin/GSK-3β-dependent signaling pathway. Here, GSK3B is linked to renal fibrosis.