OCLN and metabolic dysfunction-associated steatotic liver disease: Interestingly, the study by Lin et al. (2015b) showed that, in HFD-induced NAFLD rats, TSG (12, 24, 48 mg/kg) could improve intestinal mucosal barrier function by regulating the balance of intestinal flora as well as increasing the protein expression of ZO-1 and Occludin, and then reverse the occurrence and development of NAFLD.