Co-treatment of piperine and mitomycin C (MMC) induced apoptosis of HeLa cells along with several other biological effects such as decreased expression of p-STAT3, Bcl-2, NF-κB and increased expression of Bax, Bid, PARP activity, and caspase 3/9 and caspsae-8 induced cell apoptosis of HeLa/MMC cells. All of these biological effects of piperine exhibit its anticervical cancer potential when used along with Mitomycin C. This evidence concerns the gene CASP3 and cancer.