Treatment of KB cells for 12 h marked a high amount of ROS synthesis, which induces cell apoptosis in cancer cells. Piperine at a concentration of 100 and 200 μM leads to cell cycle arrest in the G2/M phase by 15.57 and 37.79%, respectively. Furthermore, piperine treatment also leads to induction of apoptosis by activating the caspase-3 pathway. Here, CASP3 is linked to cancer.