Piperine treatment on these cell lines can effectively activate the caspase-3-dependent apoptotic pathway. Piperine-mediated inhibition of cell growth was observed by suppressing activation of phosphorylated STAT-3 in DU145, PC-3, and LNCaP cells. Treatment with piperine can also down-regulate the expression of Nf-κB in DU145, PC-3 and LNCaP and can also induce PARP-1 cleavage. All of these processes along with inhibition of B16-F10 invasion results in the anticancer potential of piperine against prostate cancer cells. Here, STAT3 is linked to Familial prostate cancer.