We also observed that in wild-type cells, the viral genome was continually replicating from 5 h to 26 h; the relative CT values (VP1-GAPDH) were 13.46 ± 0.10, 5.38 ± 0.03, 3.53 ± 0.02, 2.79 ± 0.03 at 5, 12, 20, and 26 h post infection, respectively. The gene discussed is GAPDH; the disease is infection.