Our findings implicate specific tRNA-derived fragments as potential tumor-suppressive modulators of colorectal carcinogenesis, we identified a novel tRNAVal -derived tRF3008A that decreases the stability of the oncogenic transcript FOXK1 in colon cancer cells by binding to AGO and serving as guides in post-transcriptional silencing. Here, FBXW7 is linked to colonic neoplasm.