We analyzed the methylation indices of the promoter regions of 5 DMGs (ANKRD53, TRPV6, GATA3-AS1, SCL13A4, and CALCB) in chorionic villi trophoblasts of miscarriages with trisomy 16 and induced abortions with a normal karyotype using targeted bisulfite massive parallel sequencing (Table 2). Here, CALCB is linked to chromosome 16 trisomy.