The rescue experiments via Chamber-transwell invasion assay results revealed that CXCR4 knockdown by shRNA could effectively reduce the ERβ-enhanced lung cancer cell invasion in A549 cells (Fig. 5I), and shERβ-decreased lung cancer cell invasion could be partially blocked via treating the H1299 cells with oeCXCR4 (Fig. 5J). This evidence concerns the gene ESR2 and lung cancer.