NKAP and glioblastoma: We constructed a lentivirus-mediated RNAi targeting NKAP (shNKAP) and a lentiviral vector carrying the NKAP gene (Lv-NKAP) by transfecting to two human glioblastoma cell lines U87MG and U251, and then tested their interference effect and overexpression level by western blot (Fig. 1A, B).