IP and mass spectrometry analysis were conducted to integrate screen the proteins that can interact with HERC3, based on the results of mass spectrometry analysis, EIF5A2 possessed a higher likelihood to interact with HERC3 compared to many other proteins, moreover, EIF5A2 was previously reported to induce EMT in CRC which may better help illustrate the effects of HERC3 on EMT [25]. The gene discussed is EIF5A2; the disease is colorectal carcinoma.