To further define the role of Mll1 in the regulation of Gata4 and secretory cell differentiation, we studied the Wnt-high human colon cancer cells Ls174T and DLD1 (van de Wetering et al, 2002; van der Flier et al, 2007), from which we had previously established MLL1 knockdown lines (Grinat et al, 2020). The gene discussed is KMT2A; the disease is malignant colon neoplasm.