Then by analyzing the GO and KEGG pathways of these genes, 20 key genes related to breast cancer were finally obtained, including 19 TSGs (ACVR2A, CAV1, EGFR, FAT4, ID1, ID4, KIT, LEP, LEPR, MET, NRG1, PPARG, PRDM16, PREX2, PROX1, RYR3, SOX17, STAT5A, VIM) and 1 oncogene (PLK1). The gene discussed is ID4; the disease is breast cancer.