Then by analyzing the GO and KEGG pathways of these genes, 20 key genes related to breast cancer were finally obtained, including 19 TSGs (ACVR2A, CAV1, EGFR, FAT4, ID1, ID4, KIT, LEP, LEPR, MET, NRG1, PPARG, PRDM16, PREX2, PROX1, RYR3, SOX17, STAT5A, VIM) and 1 oncogene (PLK1). This evidence concerns the gene NRG1 and breast cancer.