The two CACNA1C loci displayed the highest methylation values in the plasma from MI patients; however, these two markers also displayed high methylation frequencies in normal plasma (25.2%, 51 of 202 for CACNA1C-1 and 28.7%, 58 of 202 for CACNA1C-2, Fig. 1c, d). Here, CACNA1C is linked to myocardial infarction.