To test if the NLRP3-GFP construct was functionally active, we used the NLRP3 reconstitution assay established previously [16], in which HEK293T cells were co-transfected with the plasmids encoding inflammasome components (NLRP3, ASC, pro-caspase 1, and pro- IL-1β) for 24 h followed by infection with Sk02 wild type (WT) for a further 18 h. Here, PYCARD is linked to infection.