PCSK9 and hyperlipidemia: Endothelium-specific deletion of Txndc5 also markedly increased eNOS expression in the ligated LCA in Txndc5∆ECKO, compared with that in control (Cdh5-Cre/ERT2), mice with hyperlipidemia induced by PCSK9 overexpression and HFD (Fig. 3E).