To investigate the regulatory effects of MIC-1 on PTP1B-related signaling pathways in non-renal cancer cells, the cancer cell lines (HCT- 116, Hep-G2, and A431) were treated with MIC-1 (0 or 4 μM) for 48 h, and then the expression of Src/Ras/Raf/ERK signaling pathway-related proteins were detected. This evidence concerns the gene SRC and renal carcinoma.